Coding

Part:BBa_K112107:Design

Designed by: Madhvi Venkatesh   Group: iGEM08_UC_Berkeley   (2008-10-21)


BglII endonuclease without stop codon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites. 

PCR mv009/mv011 on pBca9145-Bca1147    (700bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112107
-----------------------------------------------
mv009	Forward Biobricking of r.BglII!
ccataGAATTCatgAGATCTatgaagattgatataacggactataaccatgc
mv011	Reverse Biobricking of r.BglII>
cgttaGGATCCatatgtcacgattgttcctc


Source

PCR mv009/mv011 on pBca9145-Bca1147    (700bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112107

References